| 1주차 |
Introduction and team grouping |
| 2주차 |
Isolation of chromosomal DNA from bacteria |
| 3주차 |
Isolation and analysis of antibiotic resistant gene by PCR |
| 4주차 |
Cloning of PCR product using TA cloning vector |
| 5주차 |
Transformation I |
| 6주차 |
Identification of insert DNA by restriction endonuclease |
| 7주차 |
Cloning of insert DNA into expression vector |
| 8주차 |
Transformation II |
| 9주차 |
Cell disruption and protein precipitation |
| 10주차 |
Purification of His6-tagged protein using Histrap column (Affinity chromatography) |
| 11주차 |
Fast desalting |
| 12주차 |
Protein purification I (Ion exchange) |
| 13주차 |
Protein purification II (Gel filtration) |
| 14주차 |
SDS gel electrophoresis |
| 15주차 |
Final term examination |
| 16주차 |
|